The complete viral life cycle can be achieved and exclusively confined in the cells ectopically expressing SARS-CoV or SARS-CoV-2 N proteins, but not MERS-CoV N. This trVLP expresses a reporter gene (GFP) replacing viral nucleocapsid gene (N), which is required for viral genome packaging and virion assembly (SARS-CoV-2-GFP/ΔN trVLP). Here, we developed a biosafety level-2 (BSL-2) cell culture system for production of transcription and replication-competent SARS-CoV-2 virus-like-particles (trVLP). SARS-CoV-2 is classified as a biosafety level-3 (BLS-3) agent, impeding the basic research into its biology and the development of effective antivirals. Exploring these files will help you master graphing with Prism.Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes the global pandemic of COVID-19, and no effective antiviral agents and vaccines are available. Many of these portfolio graphs are linked to step-by-step instructions. When you open a portfolio graph, you'll see a brief explanation of how that graph was made, and can explore the details.
Global nonlinear regression (dose-response curves)Ĭhoose the Portfolio tab at the bottom of the Welcome dialog to browse dozens of polished graphs, each of which demonstrates several Prism features. The data table with sample data, also contains a floating note that explains how the data are arranged and gives suggestions for graphing and analyzing.įollow step-by-step examples, using sample data, for analyzing data with Prism:Īutomatic outlier elimination (exponential decay) Each sample data set comes with brief instructions, and links to longer explanations. Then follow longer detailed tours that walk you step by step through data analyses and graphing.Ĭhoose these from the Welcome dialog. Read this one page tour, which will take about five minutes to follow.
They also explain the concepts of data analysis. Both do more than explain how to use Prism. This Prism Guide is only one of three guides.
Browse the Statistics and Regression guides
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